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FIGURE 5 In vitro inhibition of oxidative stress in human alveolar epithelial cell by GHK-Cu via the upregulation of Nrf2 level After applying 5% CSE into A549 cells for 24 h, we found that the MDA level was increased, and the levels of T-AOC and GSH were decreased compared with those in A549 cells without CSE exposure, indicating that CSE stimulates an increase in oxidative stress in human alveolar epithelial cells

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To offer a semi-quantitative yet predictive understanding of the mechanism, we formulate a kinetic model for the G protein signaling and desensitization by considering essential biochemical steps from ligand binding to receptor internalization

Ethics statement The studies involving humans were approved by the Peking University Third Hospital Medical Science Research Ethics Committee